Effect of long non-coding RNA SLCO4A1-AS1 targeting microRNA-615-5p on the proliferation,apoptosis and inflammatory factors expression in esophageal cancer cells
Objective To investigate the effects of long non-coding RNA(LncRNA)solute car-rier organic anion transporter family member 4A1(SLCO4A1-AS1)targeting microRNA-615-5p(miR-615-5p)in esophageal cancer cells on cell proliferation,apoptosis,and expression of inflam-matory factors.Methods Real-time quantitative polymerase chain reaction(RT-qPCR)was used to analyze the expression of LncRNA SLCO4A1-AS1 and miR-615-5p in esophageal cancer tissues and cell lines.Eca1O9 cells were transfected with si-NC,si-LncRNA SLCO4A1-AS1,miR-NC,miR-615-5p mimic,pcDNA,pcDNA-LncRNA SLCO4A1-AS1,si-LncRNA SLCO4A1-AS1+anti-miR-NC,and si-LncRNA SLCO4A1-AS1+anti-miR-615-5p.Cell viability and apoptosis rate were meas-ured by CCK-8 and flow cytometry,respectively;cell proliferation was determined by plate cloning assay;and levels of interleukin-6(IL-6)and tumor necrosis factor-alpha(TNF-α)in the culture medium were measured using an enzyme-linked immunosorbent assay(ELISA)kit.A dual luciferase reporter gene assay was used to determine the relationship between LncRNA SLCO4A1-AS1 and miR-615-5p.Results Expression of LncRNA SLCO4A1-AS1 was upregulated,and miR-615-5p expression was downregulated in esophageal cancer tissues and cell lines.After inhibiting LncRNA SLCO4A1-AS1 expression,cell viability,the number of cell clones,and levels of IL-6 and TNF-α in the culture me-dium decreased,while miR-615-5p expression and apoptosis rate increased(P＜0.05).Compared with the miR-NC group,the miR-615-5p group showed increased miR-615-5p expression,levels of Cleaved-caspase-3 protein,and apoptosis rate,and decreased cell viability,the number of cell clones,and levels of IL-6 and TNF-α in the culture medium(P＜0.05).Compared with the si-Ln-cRNA SLCO4A1-AS1+anti-miR-NC group,the si-LncRNA SLCO4A1-AS1+anti-miR-615-5p group showed decreased miR-615-5p expression,levels of Cleaved-caspase-3 protein,and apoptosis rate,and increased cell viability,the number of cell clones,and levels of IL-6 and TNF-α in the culture medium(P＜0.05).Conclusion LncRNA SLCO4A1-AS1 can promote the occurrence and develop-ment of esophageal cancer.Inhibiting LncRNA SLCO4A1-AS1 can reduce the proliferation of esopha-geal cancer cells,decrease the expression of inflammatory factors,and induce apoptosis.
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