Expression and clinical significance of microRNA-451 in children with microcytic anemia and chronic infectious anemia
Objective To analyze the expression of microRNA-451(miR-451)in children with microcytic anemia and chronic infectious anemia,and to explore the correlation between miR-451 and erythrocyte-related parameters and its diagnostic value for anemia in children.Methods The expres-sion levels of miR-451 in serum were detected in children with microcytic anemia(n=80),chronic infectious anemia(n=80),and healthy controls(n=80)using real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).The correlation between serum miR-451 and erythrocyte-re-lated parameters[red blood cell distribution width(RDW),red blood cells(RBC),mean corpuscu-lar hemoglobin concentration(MCHC),hemoglobin(Hb),mean corpuscular hemoglobin content(MCH),mean corpuscular volume(MCV)]was analyzed using Pearson correlation analysis.The re-ceiver operating characteristic(ROC)curve was plotted to analyze the diagnostic value of miR-451 for microcytic anemia,chronic infectious anemia,and the severity of anemia.Multivariate Logistic regression analysis was used to investigate the influencing factors for the occurrence of microcytic a-nemia and chronic infectious anemia in children.Results The expression levels of serum miR-451 in the microcytic anemia group and chronic infectious anemia group were lower than those in the healthy control group(P＜0.05).The miR-451 was negatively correlated with RDW(r=-0.388,P＜0.05),and positively correlated with Hb,RBC,MCHC,MCH,and MCV(r=0.402,0.393,0.391,0.360,0.323,P＜0.05).ROC curve analysis showed that the areas under the curve of serum miR-451 for diagnosing microcytic anemia and chronic infectious anemia was 0.718 and 0.735,respectively,which was higher than that of erythrocyte-related parameters such as RDW,Hb,RBC,MCHC,MCH,and MCV(P＜0.05).Multivariate Logistic regression analysis showed that body mass index,RDW,Hb,RBC,MCHC,MCH,MCV,and miR-451 were influencing fac-tors for the occurrence of microcytic anemia and chronic infectious anemia in children(P＜0.05).With the aggravation of anemia in children,the expression level of serum miR-451 gradually de-creased(P＜0.05).ROC curve analysis showed that the level of serum miR-451 had a high diag-nostic value for the severity of anemia in children.Conclusion The expression of miR-451 is re-duced in serum of children with microcytic anemia and chronic infectious anemia,and its expression level is correlated with erythrocyte-related parameter levels.Serum miR-451 has a high diagnostic value for microcytic anemia,chronic infectious anemia,and the severity of anemia.
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维普
