Effect of miR-199a-3p/p38MAPK/NF-κB regulated by sophorae fructus flavonoids on cell proliferation,migration,invasion and secretion of inflammatory factors in breast cancer
Objective To investigate the effect of sophorae fructus flavonoids on cell prolifera-tion,migration,invasion and secretion of inflammatory factors in breast cancer.Methods The breast cancer cells BT549 were divided into normal control(NC)group,low-dose group,medium-dose group,high-dose group,miR-NC group,miR-199a-3p group,high-dose+anti-miR-NC group,and high-dose+anti-miR-199a-3p group.CCK-8 and clone formation assays were used to evaluate cell proliferation;Transwell method was performed to evaluate cell migration and invasion.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to detect the expression of miR-199a-3p.EILSA method was used to detect the levels of tumor necrosis factor-α(TNF-α),in-terleukin-6(IL-6)and interleukin-1β(IL-1β)in the cell culture supernatant.Expression levels of p-p38MAPK and p-p65 proteins were detected by Western blot.Results Compared with the NC group,the cell viability,clone formation number,migration number and invasion number of BT549 cells in the low-,medium-and high-dose groups were significantly reduced,the expression of miR-199a-3p was significantly increased,and the levels of TNF-α,IL-6 and IL-1β in the cell culture supernatant were significantly reduced(P＜0.05).Compared with the miR-NC group,the cell viability,clone formation number,migration number and invasion number of BT549 cell in the miR-199a-3p group were significantly reduced,and the levels of TNF-α,IL-6 and IL-1β in the cell culture supernatant were significantly reduced as well(P＜0.05).Compared with the high-dose+anti-miR-NC group,the cell viability,clone formation number,migration number and invasion number of BT549 in the high-dose+anti-miR-199a-3p group were significantly increased,and the levels of TNF-α,IL-6 and IL-1β in the cell culture supernatant were significantly increased as well(P＜0.05).Compared with the NC group,the expression levels of p-p38MAPK and p-p65 proteins of BT549 cells in the high-dose group were significantly reduced(P＜0.05).Compared with the high-dose+anti-miR-NC group,the expression levels of p-p38MAPK and p-p65 proteins of BT549 cells in high-dose+anti-miR-199a-3p group were significantly increased(P＜0.05).Conclusion Sophorae fructus flavonoids can inhibit cell proliferation,migration,invasion and inflammation reaction in breast cancer,and its mechanism may be achieved by up-regulating the expression of miR-199a-3p and in-hibiting p38MAPK/NF-κB pathway.
breast cancersophorae fructus flavonoidsmicroRNA-199a-3pproliferationmigrationinflammation
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