Analysis of the relationship between microRNA-15a expression and fetal heart malformation in patients with gestational diabetes mellitus
Objective To investigate the relationship between the expression of microRNA-15a(miR-15a)and fetal heart malformation in patients with gestational diabetes mellitus(GDM).Meth-ods A total of 891 patients with GDM were selected as disease group and 891 healthy pregnant women were selected as healthy group,the expression of serum miR-15a was detected and compared between the two groups.The disease group was divided into malformed group and non-malformed group according to whether fetal heart malformation occurred,serum miR-15a expression and general data of the two groups were compared.The patients in the disease group were divided into group A and group B according to the confirmed gestational age,and the patients with poor blood glucose con-trol were divided into group C and group D according to the degree of fasting blood glucose elevation.Multivariate Logistic regression analysis was used to analyze the influencing factors of fetal heart malforma-tion in GDM patients.Receiver operating characteristic(ROC)curve was drawn to analyze the predictive value of serum miR-15a expression in fetal heart malformation in GDM patients.Results Serum miR-15a expression in the disease group was significantly higher than that in the healthy group(P＜0.001).The incidence of fetal heart malformation in GDM patients was 2.87％(24/835).The proportion of serum miR-15a expression,age,pre-pregnancy body mass index,adverse pregnancy history and poor blood glucose control in the malformed group was significantly higher than that in the non-malformed group,and the proportion of regular folic acid administration in early pregnancy was significantly lower than that in the non-malformed group(P＜0.05).There was no significant difference in serum miR-15a expression and incidence of fetal heart malformation between the group A and group B(P＞0.05).The expression of serum miR-15a and the incidence of fetal heart mal-formation in the group D were significantly higher than those in the group C(P＜0.05).Serum miR-15a expression(OR=16.651,95％CI,6.252 to 44.344),age(OR=1.078,95％CI,1.006 to 1.156),poor blood glucose control(OR=3.404,95％CI,1.852 to 5.137)were the in-fluencing factors of fetal heart malformation in GDM patients(P＜0.05).The optimal critical val-ue,sensitivity,specificity and area under the curve for predicting fetal heart malformation in GDM patients were 2.34,87.50％,73.24％and 0.827(95％CI,0.799 to 0.852),respectively.Conclusion In GDM patients,serum miR-15a expression is abnormally elevated,which is an ef-fective indicator for predicting fetal heart malformation in GDM patients.
gestational diabetesmicroRNA-15afetal heart malformationglycemic control
万方数据
维普
