Circ_0114427 targets microRNA-330-5p to regulate apoptosis and inflammatory response in lipopolysaccharide-induced alveolar epithelial cells
Objective To investigate the effect of circ_0114427 on apoptosis and inflammation of alveolar epithelial cells induced by lipopolysaccharide(LPS)and its mechanism.Methods Hu-man alveolar epithelial cells were cultured in vitro and transfected with si-circ_0114427,microRNA(miR)-330-5p mimics or co-transfected with si-circ_0114427 and anti-miR-330-5p,and the cell-swere then treated with 10 mg/L LPS for 24 hours.The expression levels of circ_0114427 and miR-330-5p in the cells were detected using real-time fluorescent quantitative polymerase chain reaction(qRT-PCR).The protein levels of Cleaved-caspase3 and Cleaved-caspase9 were evaluated using western blot.Enzyme-linked immunosorbent assay(ELISA)was used to assess the expression of in-terleukin-6(IL-6)and tumor necrosis factor-alpha(TNF-α).Cell apoptosis was analyzed using flow cytometry.The interaction between circ_0114427 and miR-330-5p was validated using a dual lucifer-ase reporter assay.Results After LPS treatment of alveolar epithelial cells,the expression of circ_0114427 was upregulated,while miR-330-5p expression was downregulated in in the cells(P＜0.05).Knockdown of circ_0114427 or upregulation of miR-330-5p could inhibit LPS-induced apop-tosis,Cleaved-caspase3,Cleaved-caspase9,IL-6,and TNF-α levels in alveolar epithelial cells(P＜0.05).Circ_0114427 directly interacted with and negatively regulated miR-330-5p.Downregulation of miR-330-5p reversed the inhibitory effect of circ_0114427 knockdown on LPS-induced cell apoptosis and inflammation.Conclusion Knockdown of circ_0114427 can inhibit LPS-induced apoptosis and inflammation in alveolar epithelial cells,possibly through upregulation of miR-330-5p.
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