基于蛋白序列比对方法获得灵敏度抗体的诺如病毒免疫层析试剂条的临床应用价值
Clinical application value of an immunochromatographic test strip for Norovirus utilizing sensitivity-enhanced antibodies derived from protein sequence alignment methods
石妍 1蔡维望 2郭琛 2余久如2
作者信息
- 1. 湖北省黄石市第二医院检验科,湖北黄石,435002;黄石医养医学检验有限公司,湖北黄石,435000
- 2. 湖北省黄石市第二医院检验科,湖北黄石,435002
- 折叠
摘要
目的 探讨基于蛋白序列比对方法获得灵敏度抗体的诺如病毒免疫层析试剂条的临床应用价值.方法 选取150例疑似诺如病毒感染患者作为研究对象,回顾性分析所有患者的临床资料.所有患者均接受常规检查和优化检测试纸的诺如病毒抗原检测.利用患者临床数据对检出结果进行验证,从检出率、重复率、符合率、抗干扰能力和交叉反应等方面对该检测优化方法的检测性能进行评估.结果 优化后的抗原检测试剂条的检测准确率高于常规抗原快速检测试剂,差异有统计学意义(P＜0.05).通过评估分析发现,诺如病毒抗原检测中的一些关键因素,如样本采集时间、采集方式、保存方式等对检测结果的影响较大.通过对上述因素进行控制和管理,可以提高诺如病毒抗原检测的准确性和可靠性.结论 基于蛋白序列比对方法获得灵敏度抗体的诺如病毒试剂条的临床应用价值较高,可以优化检测方法,提高检测准确性和可靠性,减少漏诊和误诊.
Abstract
Objective To investigate the clinical application value of an immunochromatograph-ic test strip for Norovirus utilizing sensitivity-enhanced antibodies derived from protein sequence align-ment methods.Methods A retrospective analysis was conducted on a cohort of 150 suspected Noro-virus-infected patients serving as study subjects.All patients underwent routine examinations and were tested for Norovirus antigens by an optimized lateral flow immunoassay.Clinical data of all patients were reviewed to validate the test results.The diagnostic performance of this optimized detection method was evaluated in terms of detection rate,reproducibility,concordance rate,resistance to in-terference,and cross-reactivity.Results The optimized antigen test strip demonstrated significantly higher accuracy than conventional rapid antigen tests(P＜0.05).Through assessment,it was found that several key factors in Norovirus antigen detection,such as the timing of sample collection,collec-tion method,and preservation techniques,had substantial impacts on the test outcomes.By controlling and managing these factors,the accuracy and reliability of Norovirus antigen detection could be sub-stantially improved.Conclusion Clinical application of the Norovirus test strip utilizing sensitivity-en-hanced antibodies derived from protein sequence alignment methods presents high value,as it can re-fine detection methodologies,enhance both accuracy and reliability,and thereby reducing misdiagnosis and missed diagnoses.
关键词
蛋白序列比对方法/诺如病毒/抗原/检测试剂条Key words
protein sequence alignment methods/Norovirus/antigen/immunochromato-graphic test strip引用本文复制引用
基金项目
湖北省卫生健康委科研项目(WJ2023F063)
出版年
2024
国家科技期刊平台
NSTL
万方数据