Circular RNA_0032131 regulates the proliferation,migration and invasion of rheumatoid arthritis synovial fibroblasts by targeting microRNA-145-5p
Objective To investigate the effect of circular RNA_003213(hsa_circ_003213)targeting microRNA-145-5p(miR-145-5p)on the proliferation,migration and invasion of rheumatoid arthritis synovialfibroblasts(RA-FLSs).Methods The levels of hsa_circ_0032131 and miR-145-5p in peripheral blood of RA patients and healthy subjects,RA-FLSs as well as normal human synovial fibroblasts were detected.RA-FLSs were divided into control(NC)group,si-NC group,si-hsa_circ_0032131 group,miR-NC group,miR-145-5p group,anti-miR-NC+si-hsa_circ_0032131 group,anti-miR-145-5p+si-hsa_circ_0032131 group,pcDNA group and pcDNA-hsa_circ_0032131 group.CCK-8 and transwell assays were used to detect the viability,migration and invasion of RA-FLSs.The targeting relationship between hsa_circ_0032131 and miR-145-5p was determined through a dual lu-ciferase reporter assay.Results Compared with healthy subjects,the expression of hsa_circ_0032131 was increased,while the expression of miR-145-5p was decreased in the peripheral blood of RA patients,and the difference was statistically significant(P＜0.05).Compared with normal hu-man synovial fibroblasts,the expression of hsa_circ 0032131 was increased,while the expression of miR-145-5p was decreased in RA-FLSs,and the difference was statistically significant(P＜0.05).Compared with the NC group,the viability,migration and the number of RA-FLSs invasion in the si-hsa_circ_0032131 group were reduced,and the difference was statistically significant(P＜0.05).Compared with the miR-NC group,the viability,migration and the number of RA-FLSs in-vasion in the miR-145-5p group were reduced,and the difference was statistically significant(P＜0.05).Compared with the anti-miR-NC+si-hsa_circ_0032131 group,the viability,migration and the number of RA-FLSs invasion in the anti-miR-145-5p+si-hsa_circ_0032131 group were increased,and the difference was statistically significant(P＜0.05).Compared with the pcDNA group,the lev-el of miR-145-5p in the RA-FLSs of the pcDNA-hsa_circ_0032131 group was reduced(P＜0.05).The miR-145-5p was a direct target of hsa_circ_0032131.Conclusion Knockdown of hsa_circ_0032131 inhibits the proliferation,migration,and invasion of RA-FLSs by upregulating miR-145-5p.
万方数据
维普
