摘要
目的 探讨长链非编码RNA(lncRNA)牛磺酸上调基因1(TUG1)对胃癌细胞增殖、迁移和血管生成的影响.方法 基于公共数据库分析LncRNA TUG1在胃癌组织和癌旁组织中的表达水平.通过实时荧光定量聚合酶链反应(qRT-PCR)检测lncRNA TUG1在胃癌细胞系中的表达;采用si-TUGl、si-NC转染胃癌细胞SGC-7901,分别采用细胞计数试剂盒8(CCK-8)法、克隆形成实验、Transwell实验和基质胶成管实验分析敲低lncRNA TUG1对胃癌细胞增殖、集落形成、迁移和血管生成的影响.基于公共数据库分析烷基化修复蛋白B同源物5(ALKBH5)基因与lncRNA TUG1的相关性.采用放线菌素D实验验证ALKBH5与lncRNA TUG1的调控关系.通过细胞功能实验分析敲低ALKBH5对胃癌细胞增殖、集落形成、迁移和血管生成的影响.结果 lncRNA TUG1在胃癌组织和胃癌细胞系中均表达上调;敲低lncRNA TUG1后,SGC-7901细胞增殖、集落形成、迁移、血管生成能力均较对照细胞下降,差异有统计学意义(P＜0.05).数据库分析结果显示,在胃癌中,ALKBH5与lncRNA TUG1表达呈正相关(r=0.37,P＜0.05).与对照细胞相比,敲低ALKBH5的SGC-7901细胞lncRNA TUG1的RNA稳定性下降,且细胞增殖、集落形成、迁移、血管生成能力均下降,差异有统计学意义(P＜0.05).结论 ALKBH5通过诱导lncRNA TUG1表达,促进胃癌细胞的增殖、集落形成、迁移和血管生成.
Abstract
Objective To investigate the effects of long non-coding RNA(lncRNA)taurine up-regulated gene 1(TUG1)on the proliferation,migration,and angiogenesis of gastric cancer cells.Methods The expression levels of LncRNA TUG1 in gastric cancer tissues and adjacent non-cancer-ous tissues were analyzed based on public databases.The expression of lncRNA TUG1 in gastric canc-er cell lines was detected by real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR).SGC-7901 gastric cancer cells were transfected with si-TUG1 and si-NC,and the effects of knocking down lncRNA TUG1 on cell proliferation,colony formation,migration,and angiogenesis were analyzed using the Cell Counting Kit-8(CCK-8)assay,colony formation assay,Transwell assay,and Matrigel tube formation assay.The correlation between alkB homolog 5(ALKBH5)gene and lncRNA TUG1 was analyzed based on public databases.The regulatory relationship between ALKBH5 and lncRNA TUG1 was verified using Actinomycin D experiments.The effects of knocking down ALKBH5 on the proliferation,colony formation,migration,and angiogenesis of gastric cancer cells were analyzed through cell function experiments.Results LncRNA TUG1 was up-regulated in gas-tric cancer tissues and cell lines.After knocking down lncRNA TUG1,the proliferation,colony for-mation,migration,and angiogenesis abilities of SGC-7901 cells were lower than those of control cells(P＜0.05).Database analysis results showed that ALKBH5 was positively correlated with lncRNA TUG1 expression in gastric cancer(r=0.37,P＜0.05).Compared with control cells,the RNA stability of lncRNA TUG1 in SGC-7901 cells with knocked-down ALKBH5 decreased,and the cell proliferation,colony formation,migration,and angiogenesis abilities were also reduced(P＜0.05).Conclusion ALKBH5 promotes the proliferation,colony formation,migration,and angio-genesis of gastric cancer cells by inducing the expression of lncRNA TUG1.
维普
万方数据