Effects of long non-coding RNA taurine up-regulated gene 1 on the proliferation,migration and angiogenesis of gastric cancer cells
Objective To investigate the effects of long non-coding RNA(lncRNA)taurine up-regulated gene 1(TUG1)on the proliferation,migration,and angiogenesis of gastric cancer cells.Methods The expression levels of LncRNA TUG1 in gastric cancer tissues and adjacent non-cancer-ous tissues were analyzed based on public databases.The expression of lncRNA TUG1 in gastric canc-er cell lines was detected by real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR).SGC-7901 gastric cancer cells were transfected with si-TUG1 and si-NC,and the effects of knocking down lncRNA TUG1 on cell proliferation,colony formation,migration,and angiogenesis were analyzed using the Cell Counting Kit-8(CCK-8)assay,colony formation assay,Transwell assay,and Matrigel tube formation assay.The correlation between alkB homolog 5(ALKBH5)gene and lncRNA TUG1 was analyzed based on public databases.The regulatory relationship between ALKBH5 and lncRNA TUG1 was verified using Actinomycin D experiments.The effects of knocking down ALKBH5 on the proliferation,colony formation,migration,and angiogenesis of gastric cancer cells were analyzed through cell function experiments.Results LncRNA TUG1 was up-regulated in gas-tric cancer tissues and cell lines.After knocking down lncRNA TUG1,the proliferation,colony for-mation,migration,and angiogenesis abilities of SGC-7901 cells were lower than those of control cells(P＜0.05).Database analysis results showed that ALKBH5 was positively correlated with lncRNA TUG1 expression in gastric cancer(r=0.37,P＜0.05).Compared with control cells,the RNA stability of lncRNA TUG1 in SGC-7901 cells with knocked-down ALKBH5 decreased,and the cell proliferation,colony formation,migration,and angiogenesis abilities were also reduced(P＜0.05).Conclusion ALKBH5 promotes the proliferation,colony formation,migration,and angio-genesis of gastric cancer cells by inducing the expression of lncRNA TUG1.
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