Correlation between clinical staging of human immunodeficiency virus infection and specific antibody immunoblot bands
Objective To analyze the differences in the expression of specific antibodies targe-ting different human immunodeficiency virus(HIV)antigens among patients at different clinical sta-ges in Qiandongnan Prefecture of Guizhou Province,and to explore their association with the clinical staging of acquired immunodeficiency syndrome.Methods A total of 307 HIV-positive blood sam-ples from Qiandongnan of Guizhou Province were selected for specific HIV antibody immunoblotting assays.CD3+CD4+T cell counts andHIV viral load nucleic acid testing were performed on the blood samples.Multivariate regression analysis was conducted on relevant indicators during HIV in-fection and AIDS stages.Results Among the 307 HIV-infected individuals,218 were male and 89 were female,with a mean age of(48.53±16.03)years.The composition ratios of specific antibod-ies gp160,gp120,gp41,p66,p51,p31,p55,p24 and p17 were 98.7％,90.9％,92.2％,74.3％,66.4％,86.6％,3.9％,97.4％and 73.9％,respectively.Multivariate binary Logistic regression model analysis showed that expression of p24-specific antibodies were more likely to be judged as influencing factors in the infection stage(OR=0.158,95％CI,0.032 to 0.768,P＜0.05).There was a certain correlation between p24-specific antibodies and the clinical staging of HIV infection(OR=0.217,95％CI,0.005 to 0.944,P＜0.05).Using the ratio of CD3+CD4+T cell count to viral load when p24 was specifically expressed as the test variable,and the clinical stage as the state variable(AIDS stage=1,infection stage=2),a receiver operating characteristic(ROC)curve was plotted.The area under the curve was 0.653(95％CI,0.529 to 0.774,P＜0.05).Conclusion Differential expression of p24-specific antibodies may indicate that patients are in the infection stage and could potentially serve as an early warning indicator of immune function for HIV-infected individuals.
万方数据
维普
