The purpose of this study was to investigate the effect of excessive GSH on LFS,and to ex-plore the status changes after the redox balance was broken.Fresh rat LFS were extracted and cul-tured in vitro.Cell MTT test,gelatin enzyme assay,cell morphology test,scratch test,cell immuno-fluorescence staining,and western blot were employed to observe the proliferation,growth,and mi-gration of LFS with varying concentrations of GSH as well as changes in signaling pathways.The results revealed that GSH altered intracellular homeostasis of LFS,inhibited MMP-2/9 activity,and increased the expressions of focal adhesion kinase(FAK),nuclear factor kappa-B(NF-κB),phos-pho-histone gamma H2A.X(γ-H2A.X).But the excessive GSH inhibited cell migration,p38 and p53 protein expressions.In summary,excessive GSH may induce DNA damage and exhaustion in LFS cells leading to lung problems.In addition,excessive use of antioxidants can disrupt cellular homeo-stasis and may cause irreversible damage to the body.
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