负载新吲哚菁绿的还原响应性聚合物胶束增强肿瘤光动力治疗
New indocyanine green-loaded reduction responsive polymeric micelles for enhanced cancer photodynamic therapy
胡航 1肖文 1陶亚宇 1杜坤达 1袁晓音 1徐德锋1
作者信息
- 1. 常州大学药学院,江苏常州 213164
- 折叠
摘要
首先合成了二硫键连接的甲氧基聚乙二醇-胆固醇偶联物mPEG5000-SS-CH和mPEG2000-SS-CH,然后通过溶剂扩散法制备了 IR820@mPEG5000-SS-CH和IR820@mPEG2000-SS-CH这两种载新吲哚菁绿(New indocyanine green,IR820)胶束,可以显著提高IR820在生理环境下的稳定性.体外药物释放研究显示两种载IR820胶束均具有还原响应性释放特性,细胞摄取研究表明IR820@mPEG2000-SS-CH比游离IR820和IR820@mPEG5000-SS-CH具有更高的细胞摄取量,体外细胞实验显示mPEG5000-SS-CH和mPEG2000-SS-CH 具有 良好的生物相 容性,IR820@mPEG2000-SS-CH 比游离 IR820 和 IR820@mPEG5000-SS-CH具有更强的肿瘤细胞光动力杀伤作用.研究制备的IR820@mPEG2000-SS-CH为提高IR820稳定性和肿瘤光动力治疗作用提供了新的选择.
Abstract
In this study,disulfide bonded methoxypolyethylene glycol-cholesterol conjugates mPEG5000-SS-CH and mPEG2000-SS-CH were synthesized.The two types of IR820-loaded micelles,IR820@mPEG5000-SS-CH and IR820@mPEG2000-SS-CH,which might significantly improve the stability of IR820 in physiological environment,were prepared by solvent diffusion method.While vitro studies show that both mPEG5000-SS-CH and mPEG2000-SS-CH have reduction-responsive re-lease characteristics and good biocompatibility,IR820@mPEG2000-SS-CH displays enhanced cellular uptake and photodynamic killing effect on tumor cells as compared to free IR820 and IR820@mPEG5000-SS-CH.The study suggests that IR820@mPEG2000-SS-CH could be a new choice with superior stability and cancer photodynamic therapeutic effect to IR820.
关键词
新吲哚菁绿/光动力治疗/聚乙二醇/胆固醇/胶束/还原响应性Key words
new indocyanine green/photodynamic therapy/polyethylene glycol/cholesterol/micelles/reduction responsiveness引用本文复制引用
基金项目
江苏省高等学校自然科学研究面上资助项目(20KJB350005)
常州市重点研发计划资助项目(CJ20210030)
出版年
2024
国家科技期刊平台
NSTL
万方数据