Development of dried blood spot quality control materials containing 35 diagnostic markers for neonatal screening
Objective To develop dried blood spot quality control materials containing 35 diagnostic markers based on Chinese populations'blood matrix.Methods Chinese populations'blood matrix was transferred with exogenous addition of 13 amino acids,19 carnitine,succinylacetone,orotic acid and lysophosphatidylcholine 26,mixed evenly,transferred to filter papers and air-dried to yield high-and low-concentration dried blood spot quality control materials for neonatal screening.The uniformity and stability of dried blood spot quality control materials were determined using non-derivatized flow injection analysis-tandem mass spectrometry.Results The dried blood spot quality control materials had high uniformity.The concentrations of 35 diagnostic markers were within the target±3 standard deviations(SD),with a relative standard deviation of 1.01％to 16.67％.The quality control materials were stored for at least 9 days at 45 ℃,and the stability of quality control materials remained good for 6 months at-20 ℃.Conclusions The developed dried blood spot quality control materials may be used for intra-laboratory quality control in neonatal screening laboratories.
Neonatal screeningDried blood spot quality control materialTandem mass spectrometryUniformityStability
NETL
NSTL
万方数据
