Recent advances in multiplex PCR detection of pathogens
There are many kinds of pathogens causing specific syndromic infectious disease,and there may exists co-infection of pathogens.Therefore,rapid and accurate diagnosis of infectious diseases can not only effectively prevent the spread of pathogens,but also precisely guide clinical medication.Multiplex PCR(multiplex polymerase chain reaction,MPCR)technology can detect multiple pathogens simultaneously in a single reaction system,which can meet the requirements of rapid identification of pathogens in a public health emergency.MPCR technology can be divided into three types based on the detection timing of MPCR product:real-time PCR de-tection,end-point detection and post-reaction detection.This review mainly introduces these three types of MPCR technology and dis-cusses its future perspectives.
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