Molecular mechanisms of elemicin-induced lipid metabolism disorders in mice
Objective To investigate the underlying mechanisms of elemicin-induced lipid metabolism disorders in mice.Methods Mice were orally administered with elemicin at a dose of 200 mg/kg for 28 days,and serum differentially expressed metabo-lites were screened using non-target metabolomics analysis.The expression of related metabolic enzymes was detected in mouse liver and intestine specimens using quantitative real-time PCR(qPCR)assay.Results Elemicin treatment affected glycerol phospholipid,lino-leic acid and folic acid-carbon metabolism pathways,and caused serum cervonyl carnitine,phosphorylcholine and 16-hydroxyhexade-canoic acid reductions by 47％,71％and 32％in mice relative to controls.Elemicin treatment significantly up-regulated Slc22a5,Cyp4al2a and Cyp4al2b gene expression in mouse livers,Chkb gene expression in mouse ilea and Slc22a5 and Slc25a20 gene expres-sion in mouse colons,and suppressed Slc22a5,Slc25a20 and Chkbgene expression in mouse duodenums and Enpp7 gene expression in mouse colons(t=2.494 to 4.575,all P values＜0.05).Conclusions Elemicin treatment may induce lipid metabolism disorders in mice through mediatinglipid metabolism-associated gene expression and affecting glycerol phospholipid,linoleic acid and folic acid-car-bon metabolism pathways.
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