摘要
目的:探究黄芪莪术不同配伍比例中,莪术挥发油中抗肿瘤活性成分含量的变化.方法:水蒸气蒸馏法对挥发油进行提取,运用气相色谱法,测定单味莪术及黄芪莪术配伍比例为1∶1、2∶1、3∶1、5∶1、1∶2中β-榄香烯、莪术醇、吉马酮、新莪术二酮的含量.采用HP-5气相色谱柱,程序升温法,进样口温度为250℃,FID检测器温度为280℃;内标物质是水杨酸甲酯.结果:β-榄香烯、莪术醇、吉马酮、新莪术二酮4种挥发油分别在0.0030～0.1920 mg·mL-1(r=0.9969)、0.0233～2.9790 mg·mL-1(r=0.9993)、0.0059～0.3760 mg·mL-1(r=0.9994)、0.0175～1.1180 mg·mL-1(r= 0.9943)范围呈良好的线性关系,黄芪、莪术以2∶1的比例配伍时,各成分溶出量最高.结论:随着黄芪莪术配伍比例的不同,挥发油中抗肿瘤成分含量有所变化,以2∶1、3∶1的配伍比例为佳,为临床应用和药效学研究提供依据.
Abstract
Objective:To investigate the changes in the content of anti-tumor active ingredients in the volatile oil of Curcumae Rhizoma in different compatibility ratios of Astragali Radix and Curcumae Rhi-zoma.Methods:Gas chromatography was used to determine the contents of β-elemene,curcuman alcohol,gemarone and neocaridione in single Curcumae Rhizoma,Astragali Radix and Curcumae Rhizoma in the compatibility ratios of 1∶1,2:1,3:1,5:1,1:2.HP-5 gas chromatography column was used with programmed warming method,and the inlet temperature was 250℃.The temperature of the FID detector is 280℃.The sample size was 1 μL,and the standard internal substance was methyl salicylate.Results:The four volatile oils of β-olive,curcumin,gemarone and neocaridione were 0.0030-0.1920 mg·mL-1(r=0.9969),0.0233-2.9790 mg·mL-1(r=0.9993),0.0059-0.3760 mg·mL-1(r=0.9994)and 0.0175,respectively.The range of-1.1180 mg·mL-1(r=0.9943)showed a good linear relationship.When Astragali Radix and Curcumae Rhi-zoma were mixed at a 2∶1 ratio,the dissolution amount of each component was the highest.Conclusions:With the different compatibility ratios of Astragali Radix and Curcumae Rhizoma,the content of antitumor members in volatile oil has changed,and the compatibility ratio of 2∶1 and 3∶1 is the best,which provides the basis for clinical application and pharmacodynamics research.
维普
万方数据