Determination and Risk Assessment of Hexabromocyclododecane Residues in Traditional Chinese Medicine Pheretima
Objective:To establish a method for residue determination of three isomers of hexabromocyclododecanes(HBCDs)in traditional Chinese medicine Pheretima and to assess associated health risks of these emerging pollutants.Methods:The traditional Chinese medicine Pheretima powder samples were taken and added with internal standard solutions of 13C12-α-HBCD,13C12-β-HBCD,and 13C12-γ-HBCD.After ultrasonic extraction and purification with composite silica gel columns,samples were determined by high performance liquid chromatography-tandem mass spectrometry with multiple reaction monitoring for quantification of the three isomers by an internal standard method.Results:The three target HBCDs stereo-isomers exhibited good linearity(r2＞0.999),with detection and quantification limits of approximately 2pg·g-1 and 6pg·g-1,respectively.The average recovery rates of the target substances ranged from 97％to 120％,with RSDs from 7.1％to 7.4％,and precisions between 1.1％and 7.4％(n=6).Out of 78 batches of Pheretima,HBCDs were detected in 73 batches,giving a detection rate of 94％,with a-HBCD being the most abundant congener.Risk assessment of HBCDs in Pheretima using the Margin of Exposure(MOE)approach recommended by the European Food Safety Authority(EFSA)indicated that the risk of health issues arising from the intake of HBCDs through Pheretima wss minimal.Conclusion:The established method is sensitive,accurate,and reliable.It is suitable for the quantitative analysis of HBCDs residues in Pheretima and can provide a scientific reference for the safety monitoring of exogenous emerging pollutants in traditional Chinese medicine.
PheretimaTraditional Chinese MedicineHexabromocyclododecanesResiduesHPLC-MS/MSRisk Assessment
NETL
NSTL
万方数据
