Inhibitory Effects of Carvacrol on Biofilms of Carbapenem-resistant Kleb-siella Pneumoniae in vitro
Objective:To investigate carvacrol on its anti-biofilm activity against carbapenem-resistant Klebsiella pneumoniae(CRKP)in vitro,and its combined effects with meropenem and levofloxacin.Meth-ods:Minimum inhibition concentrations(MIC)of imipenem and meropenem against 10 clinically isolated Klebsiella pneumoniae were detected by a broth micro-dilution method.MIC and minimal bactericidal con-centration(MBC)of carvacrol were determined by broth micro-dilution.The effects of carvacrol on the growth of CRKP were determined by growth curves.Its effect on CRKP biofilm formation was determined by crystal violet staining.The minimum biofilm inhibition concentration(MBIC)and minimum biofilm eradi-cation concentration(MBEC)were determined by the broth micro-dilution method.The fractional biofilm inhibition concentrations(FBICs)were determined by a microdilution checkerboard method.Results:The MIC and MBC of carvacrol against two strains of CRKP in vitro were 156μg·mL-1 and 313 μg·mL-1.The MBIC and MBEC of carvacrol against biofilm of CRKP1 were 313μg·mL-1 and 1250 μg·mL-1,respectively.Carvacrol significantly reduced the growth and biofilm formation abilities of CRKP.FBICs of carvacrol in combination with meropenem and levofloxacin were 0.31 and 0.37,respectively.Conclusion:Carvacrol have antibacterial and antibiofilm activities against CRKP in vitro,and carvacrol combine with meropenem and levofloxacin have synergistic antibiofilm effects on CRKP,which provides a new idea for the treatment of CRKP infection.
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