Determination of Related Substances of Lycorine Thiolate Hydrochloride for Injections by HPLC
Objective:A liquid chromatography(HPLC)method was developed to determine related sub-stances in lycorine thiolate hydrochloride for injections.Methods:A chromatographic column of Kromasil C18(4.6 mm×250 mm,5 μm)was used for the HPLC method.Phosphate buffer was used as mobile phase A and acetonitrile as mobile phase B for gradient elution.The flow rate was 1.2 mL·min-1 with column tem-perature contained at 40℃.The detection wavelength was 290 nm.The injection volume was 10 μL.Re-sults:Lycorine thiolate hydrochloride and its impurities were effectively separated in this method.Good linearity were obtained in the ranges of LOQ concentrations to 200％limit concentrations.The average re-coveries among the low,middle and high concentrations were from 96.8％to 103.6％.Conclusion:This method has the advantages of high accuracy,good reproducibility,simplicity and rapidity,which can be used for the quality control of lycorine thiolate hydrochloride for injections.
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