目的:建立司美格鲁肽非法添加的专属筛查检测方法。方法:采用Fortis C18色谱柱(2。1 mm × 50 mm，1。7 μm)，以0。2％甲酸-乙腈为流动相，超高压液相色谱梯度洗脱分离，ESI正离子化三重四极杆串联质谱法检测。利用色谱保留时间、一级质谱母离子及其二级质谱特征碎片三方面信息，对非法添加的司美格鲁肽进行定性和定量测定。结果:司美格鲁肽质量浓度在25～500ng·mL-1范围内线性关系良好。结论:该方法准确、快速，可应用于司美格鲁肽非法添加的检测。
Rapid Detection of Illegally Added Semaglutide by UPLC-MS/MS Method
Objective:To establish a specific screening detection method for the illegal addition of semaglutide.Methods:Using a Fortis C18 column(2.1 mm × 50 mm,1.7 μm)and gradient 0.2％formic acid-acetonitrile elution for separation,ultra-high pressure liquid chromatography was performed.ESI positive ionization triple quadrupole tandem mass spectrometry was used for detection.Qualitative and quantitative determination was conducted using information from three aspects:chromatographic retention time,primary mass spectrometry of parent ion,and secondary mass spectrometry of characteristic fragments.Results:Semaglutide was linearly detected in the range of 25-500 ng·mL-1.Conclusion:This method is accurate,fast and exclusive;it can be applied to the detection of illegally added semaglutide.
SemaglutideIllegal additionUltra-high performance liquid chromatography-triple quadrupole mass spectrometry
万方数据
维普
