Elucidating regulatory processes of intense physical activity by multi-omics analysis

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维普
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外文摘要：Background:Physiological and biochemical processes across tissues of the body are regulated in response to the high demands of intense physical activity in several occupations,such as firefighting,law enforcement,military,and sports.A better understanding of such processes can ultimately help improve human performance and prevent illnesses in the work environment.Methods:To study regulatory processes in intense physical activity simulating real-life conditions,we performed a multi-omics analysis of 3 biofluids(blood plasma,urine,and saliva)collected from 11 wildland firefighters before and after a 45 min,intense exercise regimen.Omics profiles post-vs.pre-exercise were compared by Student's t-test followed by pathway analysis and comparison between the different omics modalities.Results:Our multi-omics analysis identified and quantified 3835 proteins,730 lipids and 182 metabolites combining the 3 different types of samples.The blood plasma analysis revealed signatures of tissue damage and acute repair response accompanied by enhanced carbon metabolism to meet energy demands.The urine analysis showed a strong,concomitant regulation of 6 out of 8 identified proteins from the renin-angiotensin system supporting increased excretion of catabolites,reabsorption of nutrients and maintenance of fluid balance.In saliva,we observed a decrease in 3 pro-inflammatory cytokines and an increase in 8 antimicrobial peptides.A systematic literature review identified 6 papers that support an altered susceptibility to respiratory infection.Conclusions:This study shows simultaneous regulatory signatures in biofluids indicative of homeostatic maintenance during intense physical activity with possible effects on increased infection susceptibility,suggesting that caution against respiratory diseases could benefit workers on highly physical demanding jobs.

外文关键词：

Multi-omics analysisIntense exerciseHuman performanceBiofluidsMetabolismImmunity

作者：

Ernesto S.Nakayasu、Marina A.Gritsenko、Young-Mo Kim、Jennifer E.Kyle、Kelly G.Stratton、Carrie D.Nicora、Nathalie Munoz、Kathleen M.Navarro、Daniel Claborne、Yuqian Gao、Karl K.Weitz、Vanessa L.Paurus、Kent J.Bloodsworth、Kelsey A.Allen、Lisa M.Bramer、Fernando Montes、Kathleen A.Clark、Grant Tietje、Justin Teeguarden、Kristin E.Burnum-Johnson

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作者单位：

Biological Sciences Division,Pacific Northwest National Laboratory,Richland,WA 99352,USA

Environmental and Molecular Sciences Division,Pacific Northwest National Laboratory,Richland,WA 99352,USA

Centers for Disease Control and Prevention,National Institute for Occupational Safety and Health,Western States Division,Denver,CO 80204,USA

Computational Analytics Division,Pacific Northwest National Laboratory,Richland,WA 99352,USA

National Security Directorate,Pacific Northwest National Laboratory,Richland,WA 99352,USA

Los Angeles County Fire Department,Los Angeles,CA 90063,USA

Centers for Disease Control and Prevention,National Institute for Occupational Safety and Health,Respiratory Health Division,Morgantown,WV 26505,USA

Environmental and Molecular Toxicology,Oregon State University,Corvallis,OR 97331,USA

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基金：

Department of Energy(DOE)Office of Science User Facility sponsored by the Biological and Environmental Research(BER)programBRAVE Agile Investment from the PNNL

项目编号：

DE-AC05-76RL01830

出版年：

2024

DOI：

10.1186/s40779-023-00477-5

军事医学研究(英文)

CSTPCD

ISSN：2095-7467

年,卷(期)：2024.11(4)