The changes of adiponectin receptor mRNA expressions and lipidosome phagocytosis of macrophages after stimulated by adiponectin-intervened TNF-α
Objective To investigate the effects of TNF-α on adiponectin receptor mRNA expressions and lipid phagocytosis of macrophages, and its further response to the interference effects of adiponectin. Methods THP-1 cells were induced and differentiated into macrophages by phorbol ester. RT-PCR was used to detect the expression of adiponectin receptor mRNA of the macrophages, which were stimulated with different concentrations of TNF-α (0.1,1,10,100 ng/ml) for 24 h,and TNF-α 0 ng/ml was set as the control. Macrophages that stimulated with TNF-α 0 ng/ml and 10 ng/ml were further cultured ia growth-promoting media containing 0. 2 mg/ml LDL for 24 h, intracellular fat accumulation was observed using oil red O staining and quantified by colorimetry. Meanwhile the macrophages were further interfered with 20 μg/ml adiponectin, and adiponectin receptor mRNA expressions and lipidosome phagocytosis of macrophages were tested Results The inhibition of adiponectin receptor mRNA expression in the macrophages increased as the concentration of TNF-α was increasing(P<0. 05). When the macrophages were stimulated with TNF-α 10 ng/ml, the accumulation of lipid within the cells was obviously increased compared to TNF-α 0 ng/ml (P<0. 05). After the macrophages were further interfered with adiponectin, adiponectin receptor mRNA expression was up-regulated and the accumulation of lipid within the cells was decreased (P<0. 05). Conclusion Adiponectin and adiponectin receptor play an important role in regulating lipid phagocytosis of macrophages, which may be a new target for the prevention or treatment of atherosclerosis.
NETL
NSTL
万方数据
维普
