Objective To investigate the effects and underlying mechanism of serine protease inhibitor B7(SERPINB7)on the proliferation,apoptosis,migration and invasion of breast cancer cells.Methods Human breast cancer cell line MCF-7 was divided into three groups of sh-SERPINB7(transfected with SERPINB7 RNA interference lentivirus),BC(without treatment)and NC(transfected with negative control lentivirus).The mRNA expression of SERPINB7 was measured by qRT-PCR The proliferation,apoptosis,migration and invasion of MCF-7 cells were determined by CCK-8,flow cytometry,cell scratch and Transwell assays,respectively.The protein expressions of SERPINB7,Bax,Bcl-2,Snail,Slug,TGF-β,Smad3 and p-Smad3 were detected by Western blot.Results The protein expression of SERPINB7 in MCF-7 cells was higher than that in human mammary epithelial cell line MCF-10A(P＜0.05).Compared with groups of BC and NC,the mRNA expression of SERPINB7 and protein expressions of Bcl-2,Snail,Slug,TGF-β and p-Smad3 were downregulated,rate of cell apoptosis was increased,healing rate of cell scratch and number of invasive cells were decreased,and protein expression of Bax was upregulated in group sh-SERPINB7(P＜0.05).Conclusion SERPINB7 is highly expressed in breast cancer cells.Knockdown of SERPINB7 may inhibit the migration and invasion and promote apoptosis of breast cancer cells by inhibiting TGF-β/Smad3 signaling pathway.
Breast cancerSerine protease inhibitor B7Cell migrationCell invasion
维普
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