Effects of SERPINB7 on proliferation,apoptosis,migration and invasion of breast cancer cells
Objective To investigate the effects and underlying mechanism of serine protease inhibitor B7(SERPINB7)on the proliferation,apoptosis,migration and invasion of breast cancer cells.Methods Human breast cancer cell line MCF-7 was divided into three groups of sh-SERPINB7(transfected with SERPINB7 RNA interference lentivirus),BC(without treatment)and NC(transfected with negative control lentivirus).The mRNA expression of SERPINB7 was measured by qRT-PCR The proliferation,apoptosis,migration and invasion of MCF-7 cells were determined by CCK-8,flow cytometry,cell scratch and Transwell assays,respectively.The protein expressions of SERPINB7,Bax,Bcl-2,Snail,Slug,TGF-β,Smad3 and p-Smad3 were detected by Western blot.Results The protein expression of SERPINB7 in MCF-7 cells was higher than that in human mammary epithelial cell line MCF-10A(P＜0.05).Compared with groups of BC and NC,the mRNA expression of SERPINB7 and protein expressions of Bcl-2,Snail,Slug,TGF-β and p-Smad3 were downregulated,rate of cell apoptosis was increased,healing rate of cell scratch and number of invasive cells were decreased,and protein expression of Bax was upregulated in group sh-SERPINB7(P＜0.05).Conclusion SERPINB7 is highly expressed in breast cancer cells.Knockdown of SERPINB7 may inhibit the migration and invasion and promote apoptosis of breast cancer cells by inhibiting TGF-β/Smad3 signaling pathway.
Breast cancerSerine protease inhibitor B7Cell migrationCell invasion
万方数据
维普
