Effect of heart valve replacement surgery on pyridoxal 5'-phosphate dependent detection of serum ALT and AST activities
Objective To explore the effect of heart valve replacement(HVR)surgery on pyridoxal 5'-phosphate dependent detection of serum ALT and AST activities.Methods Serum samples were collected from 22 patients undergoing single valve HVR at the time points of before surgery,immediately after surgery and on the 1st,2nd,3rd day after surgery.Serum ALT and AST activities were measured using VITROS 5600 dry biochemical analyzer(dry slice method)and Beckman Coulter AU5800 fully automatic biochemical analyzer(reagent method),respectively.The measurement bias values for detecting ALT and AST activities by the two methods were calculated.The blood phosphorus concentration of the patients was measured as well.Results The preoperative liver and kidney function indicators of 22 HVR patients were within the normal ranges.The measurement bias of ALT activity on the 2nd and 3rd day was higher than that on the 1st day after surgery(P＜0.05).The measurement bias of AST activity on the 3rd day after surgery was higher than that at the times of immediately and on the 1st day after surgery(P＜0.05).The measurement bias of ALT activity on the 3rd day after surgery was higher than that on the 1st day after surgery in female patients(P＜0.05).The blood phosphorus concentration was lower after operation than that before(P＜0.05),which on the 1st day after surgery was lower than that at the other time points(P＜0.05).Conclusion The increased bias of postoperative ALT viability measurement in HVR patients may be related to low concentrations of serum phosphorus.The more accurate serum ALT activity level can be obtained with the dry slice method pyridoxal 5'-phosphate dependent test,which is helpful for clinical monitoring of disease changes.
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