Objective To investigate the effects of reactive oxygen species-responsive nanoparticles(OxbCD NP)on the oxidative stress and expressions of inflammatory factors in mice.Methods Twenty mice were equally randomized into four groups of normal control group,toe swelling model group,OxbCD NP 1.0 mg/kg group and OxbCD NP 10 mg/kg group,and the effect of OxbCD NP on the toe thickness of mice was observed.Twenty mice were equally randomized into four groups of normal control group,acute peritonitis model group,OxbCD NP 0.1 mg/kg group and OxbCD NP 1.0 mg/kg group,and the levels of H2O2,TNF-α and IL-1β in the peritoneal lavage fluid were detected by ELISA method.Eighteen mice were equally randomized into three groups of normal control group,intraperitoneal OxbCD NP 1 000 mg/kg group and intravenous OxbCD NP 1 000 mg/kg group,and the effects of large dose of OxbCD NP on the blood routine and hepatorenal function were observed.Results Compared with toe swelling model group,the toe thickness was decreased in OxbCD NP 10 mg/kg group(P＜0.01).Compared with acute peritonitis model group,the levels of TNF-α and IL-1β were decreased in OxbCD NP 0.1 mg/kg group(P＜0.01),and the levels of H2O2,TNF-α and IL-1β were all decreased in OxbCD NP 1.0 mg/kg group(P＜0.01).The levels of ALT,AST,serum urea,SCr,Hb,RBC,WBC and Plt in groups of intraperitoneal OxbCD NP 1 000 mg/kg and intravenous OxbCD NP 1 000 mg/kg were not different from those in normal control group(P＞0.05).Conclusion OxbCD NP has good antioxidative and anti-inflammatory effects with higher biosafety in vivo.
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