Objective To analyze the factors influencing perinatal red blood cell transfusion of the Rh(D)negative puerperae.Methods The clinical data of 301 Rh(D)negative puerperae were analyzed retrospectively,of whom 60 cases were given red blood cell transfusion(group A,)and 241 cases did not(group B).Basic maternal data and laboratory test indicators were analyzed with multivariate logistic regression analysis.Results The gestational age and proportions of twin pregnancy,prenatal Hb<100 g/L,scar uterus,Cesarean section,placenta previa,postpartum blood loss>1 000 mL were significantly different between the two groups(P<0.05).Multivariate logistic regression analysis showed that prenatal Hb<100 g/L(OR=29.031,P<0.01),Cesarean section(OR=13.074,P<0.01)and postpartum blood loss>1 000 mL(OR=23.756,P<0.01)were the independent risk factors for perinatal red blood cell transfusion of the Rh(D)negative puerperae.A total of 219 units autologous blood were stored from 180 puerperae before delivery.Autologous blood re-transfusion rate was 15.56%.Conclusion Prenatal Hb<100 g/L,Cesarean section and postpartum blood loss>1 000 mL are the independent risk factors of perinatal red blood cell transfusion in Rh(D)negative puerperae.For Rh(D)negative puerperae with high risk of bleeding,implementation of pre-deposit autologous blood donation can not only alleviate the shortage of allogeneic blood but also safeguard transfusion safety.