Tanshinone ⅡA promotes wound repair of zebrafish caudal fin
Objective To investigate the effect and underlying mechanism of tanshinone Ⅱ A on the wound repair of zebrafish caudal fin.Methods The tails of 48 wild or transgenic zebrafishes were cut for establishing the caudal fin wound model,which then were equally divided into four groups of A(model control),B1(treated with tanshinone Ⅱ A 50 μg/mL),B2(treated with tanshinone Ⅱ A 100μg/mL)and B3(treated with tanshinone Ⅱ A 200 μg/mL).Another 12 wild or transgenic zebrafishes without tail cutting were taken as the blank controls(group C).The regeneration length of the caudal fin was recorded after culture for 3 days.The number of neutrophils in the regenerated zebrafish caudal fin was observed.The effect of tanshinone Ⅱ A on the content of ROS,cell apoptosis and mRNA expressions of TNF-α and IL-6 in the regenerated zebrafish caudal fin were analyzed.Results The regeneration length of caudal fin,number of neutrophils,content of ROS,number of cell apoptosis and mRNA expressions of TNF-α and IL-6 in group A were higher than those in group C(P<0.05).Compared with group A,the regeneration length of caudal fin was increased,while the number of neutrophils,content of ROS,number of cell apoptosis and mRNA expressions of TNF-α and IL-6 were decreased in a dose-dependent manner in groups of B1,B2 and B3(P<0.05).Conclusion TanshinoneⅡA can clear the inflammatory cells,inhibit the secretion of inflammatory factors,and promote the wound repair of zebrafish caudal fin.