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丹参酮ⅡA促进斑马鱼尾鳍创面修复

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目的 探讨丹参酮ⅡA对斑马鱼尾鳍创面修复的作用及其可能机制.方法 取48条野生型或转基因斑马鱼切尾构建尾鳍创伤模型后,随机均分为模型组及低浓度丹参酮ⅡA(50 μg/mL)、中浓度丹参酮Ⅱ A(100 μg/mL)和高浓度丹参酮Ⅱ A(200 μg/mL)组;另取未切尾的12条野生型或转基因斑马鱼作为对照组.培养3 d后记录野生型斑马鱼尾鳍再生长度,观察转基因斑马鱼再生尾鳍中性粒细胞数量,并分析丹参酮Ⅱ A对野生型斑马鱼再生尾鳍中活性氧(ROS)含量、细胞凋亡及TNF-α和IL-6 mRNA表达的影响.结果 模型组尾鳍再生长度、中性粒细胞数量、ROS含量、细胞凋亡数以及TNF-α和IL-6 mRNA表达均较对照组增加(P<0.05);与模型组相比,低浓度丹参酮ⅡA组、中浓度丹参酮ⅡA组、高浓度丹参酮ⅡA组尾鳍再生长度呈浓度依赖性地增加,而中性粒细胞数量、ROS含量、细胞凋亡数以及TNF-α和IL-6 mRNA表达呈浓度依赖性地减少(P<0.05).结论 丹参酮ⅡA可清除炎症细胞,抑制炎症因子分泌,从而促进斑马鱼尾鳍创面修复.
Tanshinone ⅡA promotes wound repair of zebrafish caudal fin
Objective To investigate the effect and underlying mechanism of tanshinone Ⅱ A on the wound repair of zebrafish caudal fin.Methods The tails of 48 wild or transgenic zebrafishes were cut for establishing the caudal fin wound model,which then were equally divided into four groups of A(model control),B1(treated with tanshinone Ⅱ A 50 μg/mL),B2(treated with tanshinone Ⅱ A 100μg/mL)and B3(treated with tanshinone Ⅱ A 200 μg/mL).Another 12 wild or transgenic zebrafishes without tail cutting were taken as the blank controls(group C).The regeneration length of the caudal fin was recorded after culture for 3 days.The number of neutrophils in the regenerated zebrafish caudal fin was observed.The effect of tanshinone Ⅱ A on the content of ROS,cell apoptosis and mRNA expressions of TNF-α and IL-6 in the regenerated zebrafish caudal fin were analyzed.Results The regeneration length of caudal fin,number of neutrophils,content of ROS,number of cell apoptosis and mRNA expressions of TNF-α and IL-6 in group A were higher than those in group C(P<0.05).Compared with group A,the regeneration length of caudal fin was increased,while the number of neutrophils,content of ROS,number of cell apoptosis and mRNA expressions of TNF-α and IL-6 were decreased in a dose-dependent manner in groups of B1,B2 and B3(P<0.05).Conclusion TanshinoneⅡA can clear the inflammatory cells,inhibit the secretion of inflammatory factors,and promote the wound repair of zebrafish caudal fin.

Tanshinone ⅡAZebrafishWound repairInflammatory response

马柏强、宋承骏、叶小荣

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323000 浙江,丽水市人民医院急诊创伤外科

丹参酮ⅡA 斑马鱼 创面修复 炎症反应

丽水市科技计划

2021SJZC027

2024

江苏医药
江苏省人民医院(南京医科大学第一附属医院)

江苏医药

影响因子:0.707
ISSN:0253-3685
年,卷(期):2024.50(5)
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