Effect of phycoerythrin on growth and apoptosis of HeLa cells
Objective To investigate the effect of phycoerythrin(PE)on the growth and apoptosis of HeLa cells and underlying mechanism.Methods High purity PE was obtained from asparagus.Cervical cancer HeLa cells were treated with PE in different concentrations of 0,5,10,20 and 40 mg/L.The cell growth was detected by MTT assay,cell nucleus apoptosis was detected by Hoechst 33342 staining,and cell apoptosis was detected by flow cytometry.The mitochondrial membrane potential was detected by cell membrane potential probe JC-1 staining.The apoptosis-related genes and proteins were detected by real-time fluorescence quantitative PCR and Western blot.Results Compared with PE 0 mg/L,PE 10,20 and 40 mg/L inhibited HeLa cell growth,increased late apoptosis rate and total apoptosis rate,increased mRNA expressions of Caspase-3,Caspase-9 and Bax,and cleaved Caspase-3 protein expression in a dose-dependent manner(P＜0.05).Compared with PE 0 mg/L,PE 20 and 40 mg/L increased cell nuclear apoptosis rate after treatment for 24 hours,and PE 10,20 and 40 mg/L increased cell nuclear apoptosis rate after treatment for 48 hours,which was more obvious at 48 hours than 24 hours(P＜0.05).Compared with PE 0 and 10 mg/L,PE 20 and 40 mg/L decreased mitochondrial membrane potential of HeLa cells,downregulated Bcl-2 mRNA and protein expressions,and upregulated CytC protein expression,which were more obvious after treated with PE 40 mg/L than those treated with PE 20 mg/L(P＜0.05).Conclusion Different concentrations of PE can inhibit the growth of HeLa cells and induce cell apoptosis,which may be related to the reduction of mitochondrial membrane potential and the regulation of protein expressions associated with mitochondrial apoptosis pathway.
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