Effect of Fujie Huayu granules on cardiac function of sepsis mice through regulating mitochondria autophagy pathway
Objective To explore the effect of Fujie Huayu granules on cardiac function of sepsis mice through regulating mitochondria autophagy pathway.Methods Forty-five male C57BL/6 mice were randomly divided into five groups with 9 mice each.The sepsis models were established in groups of A,B,M and C by cecal ligation and perforation.Group S was taken as sham operation group.Group A and group B were given gavage of Fujie Huayu granules 2 mL·mg-1·kg-1 at 6 and 12 hours after model establishment,respectively.The autophagy inhibitor 3-methyladenine 2 mg/kg was injected via the tail vein in group C at 6 hours later,and the same amount of normal saline was injected in groups of M and S at 6 hours later.After 24 hours,the cardiac function was evaluated by hemodynamic indicators,autophagy-related protein expression was detected by Western blot,myocardial histopathological changes were observed by HE staining,and myocardial apoptosis was detected by TUNEL staining in the five groups.Results Compared with group S,the hemodynamic indicators of groups of A,B,C and M were worse,expressions of autophagy-related proteins were decreased,and apoptosis rate of cardiomyocytes was increased(P＜0.05).Compared with group M,the hemodynamic indicators were improved,expression of autophagy-related proteins was increased,apoptosis rate of cardiomyocytes was decreased in groups of A and B,but hemodynamic indicators were worse,expression of autophagy-related proteins was decreased and apoptosis rate of cardiomyocytes was increased in group C(P＜0.05).The improvements of the above indicators in group A were better than those in group B(P＜0.05).Conclusion Fujie Huayu granules may improve cardiac dysfunction,reduce myocardial injury and reduce myocardial cell apoptosis in sepsis mice through regulating mitochondria autophagy pathway.
SepsisFujie Huayu granulesMitochondrial autophagyCardiac function
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