Effect of water extract of seeds of Descurainia sophia(L.)Webb ex Prantl through regulating Fas/FasL pathway on myocardial cells in rats with heart failure
Objective To investigate the effect of water extract of seeds of Descurainia sophia(L.)Webb ex Prantl through regulating Fas/Fas ligand(FasL)pathway on myocardial cells in rats with heart failure.Methods The heart failure model was established with abdominal aortic coarction method in 20 rats,which then were divided into two groups with 10 rats each.Group A was intragastrically given water extract of seeds of Descurainia sophia(L)Webb ex Prantl 10 g·kg-1·d-1 and group M was given normal saline 5 mL·kg-1·d-1.Ten rats accepted sham operation(group S)were intragastrically given normal saline 5 mL·kg-1·d-1.After 6 weeks,the body weight,heart weight,heart coefficient,cardiac function indicators and hemodynamic indicators were compared among the three groups.The myocardial histomorphology was observed by HE staining and Fas/FasL pathway-related protein expressions were detected by Western blot.Results Compared with group S,the heart weight and heart coefficient of rats were increased(P＜0.05),cardiomyocytes were hypertrophy,fibrous tissues were disordered and cell space was enlarged,and Fas/FasL pathway-related protein expressions were increased in group M(P＜0.05).Compared with group M,heart weight and heart coefficient of rats were decreased(P＜0.05),cardiac function indicators,hemodynamic indicators and myocardial histomorphology were improved(P＜0.05),and Fas/FasL pathway-related protein expressions were decreased in group A(P＜0.05).Conclusion The water extract of seeds of Descurainia sophia(L.)Webb ex Prantl alleviates the damage of cardiomyocytes in rats with heart failure by regulating Fas/FasL pathway,therefore improving the symptoms of heart failure.
Seeds of Descurainia sophia(L.)Webb ex PrantlHeart failureFas/Fas ligand pathway
万方数据
维普
