动态对比增强MRI参数与三种方法评估食管癌组织中PD-L1蛋白阳性表达的关系
Relationship between dynamic contrast-enhanced MRI parameters and positive expression of PD-L1 protein evaluated by three methods in esophageal cancer tissues
严建 1李丹明1
作者信息
- 1. 210029 江苏,南京医科大学第一附属医院放疗科
- 折叠
摘要
目的 探讨动态对比增强MRI(DCE-MRI)参数与三种方法评估食管癌组织中程序性死亡配体1(PD-L1)蛋白阳性表达的关系.方法 23例食管癌患者行DCE-MRI检查,获取DCE-MRI参数[转运常数(Ktrans)、速率常数(Kep)和容积分数(Ve)].食管癌组织标本采用链霉菌抗生物素蛋白-过氧化物酶连结法进行免疫组织化学染色,分别采用三种方法[肿瘤细胞阳性比例评分(TPS)、联合阳性评分(CPS)和免疫细胞阳性评分(ICS)]评估PD-L1阳性表达,分析三种方法评估食管癌组织中PD-L1蛋白阳性表达与DCE-MRI参数的相关性.结果 食管癌患者Ktrans为0.20(0.16,0.27)min-1,Kep为 0.42(0.29,0.51)min-1,Ve为 0.46(0.36,0.62).TPS、CPS 和 ICS评估 PD-L1 蛋白阳性表达率分别为26.1％、34.8％和47.8％.TPS评估中PD-L1蛋白阳性表达组Ktrans升高,而Kep降低(P＜0.05).CPS评估中PD-L1蛋白阳性表达组Kt_升高,而Ve降低(P＜0.05).评估食管癌组织PD-L1蛋白阳性表达的CPS与DCE-MRI参数Ktrans呈负相关(P＜0.05),与Ve呈正相关(P＜0.05);评估食管癌组织PD-L1蛋白阳性表达的TPS与DCE-MRI参数Ktrans和Kep呈正相关(P＜0.05).结论 DCE-MRI参数与TPS和CPS评估食管癌组织PD-L1蛋白阳性表达关系密切.
Abstract
Objective To investigate the relationship between dynamic contrast-enhanced MRI(DCE-MRI)parameters and positive expression of programmed death-ligand 1(PD-L1)protein evaluated by three methods in esophageal cancer tissues.Methods Twenty-three patients with esophageal cancer underwent DCE-MRI examination,and the DCE-MRI parameters of Ktrans,Kep and Ve were obtained.Streptomyces antibiotin protein-peroxidase binding method was used for immunohistochemical staining of esophageal cancer tissue samples,and positive expression of PD-L1 protein was evaluated by three methods of tumor positive score(TPS),combined positive positive score(CPS)and immune cell score(ICS).Correlations of DCE-MRI parameters with positive expression of PD-L1 protein by three methods in esophageal cancer tissues were analyzed.Results Patients with esophageal cancer were with Ktrans 0.20(0.16,0.27)min-1,Kep 0.42(0.29,0.51)min-1 and Ve 0.46(0.36,0.62).The positive expression rates of PD-L1 protein evaluated by TPS,CPS and ICS were 26.1％,34.8％and 47.8％,respectively.Ktrans was increased,while Kep was decreased in PD-L1 protein positive expression group evaluated by TPS(P＜0.05).Ktrans was increased,while Ve was decreased in PD-L1 protein positive expression group evaluated by CPS(P＜0.05).PD-L1 protein positive expression evaluated by CPS was negatively correlated with Ktrans(P＜0.05),and positively correlated with Ve(P＜0.05).PD-L1 protein positive expression evaluated by TPS was positively correlated with Ktrans and Kep(P＜0.05).Conclusion DCE-MRI parameters are closely related to positive expression of PD-L1 protein in esophageal cancer tissues evaluated by TPS and CPS.
关键词
动态对比增强磁共振成像/程序性死亡配体1/食管癌Key words
Dynamic contrast-enhanced magnetic resonance imaging/Programmed death-ligand 1/Esophageal cancer引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据