Effect of lutein on gene expressions in senescent cells based on RNA sequencing
Objective To analyze the effect of lutein on the gene expressions in senescent cells based on RNA sequencing.Methods The 55th generation human embryonic lung diploid fibroblast 2BS cells were treated with lutein 1 μmol/L(group A)or 0.1％DMSO(group B)for 48 hours.RNA was extracted and sequenced to screen out differentially expressed genes,and signal pathways with significant differences were analyzed.Results Compared with group B,133 genes were differentially expressed in group A,of which 83 genes were up-regulated and 50 genes were down-regulated.Gene ontology enrichment analysis showed that differentially expressed genes were mainly enriched in transcriptional regulation,DNA template,signal transduction,immune process and other biological processes,protein binding,nucleotide binding,transferase activity and other active molecular functions,as well as cell membrane,exosome,plasma membrane and other cell parts.Kyoto encyclopedia of genes and genomes enrichment analysis showed that the main enrichment pathways were relaxin signaling pathway,Ras family small molecule G protein signaling pathway,NF-κB signaling pathway,and advanced glycosylation end-product/advanced glycosylation end-product receptor signaling pathway.Conclusion Lutein can significantly regulate the expression of some genes in senescent cells,and is related to inflammatory signaling pathways.Lutein may play an anti-aging role by regulating inflammatory response.
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