Bioinformatic, gene expression and enzyme activity analyses of manganese peroxidases in Volvariella volvacea
We identified four putative manganese peroxidases (MnP) in the genome of the straw mushroom Volvariella volvacea,and further analyzed their bioinformatic traits.The mnp gene expression and enzyme activity analyses were also conducted in this study.The upstream region of mnp genes of V.volvacea contains putative AP-2,cAMP,MRE,XRE and HSE response elements,which are known to be involved in regulation of carbon,nitrogen,heavy metal ions and aromatic compounds.Each MnP protein has a signal peptide but without transmembrane helices,suggesting they are the secretory proteins.MnP protein sequences all contain the conserved sites related to Mn2+ and Ca2+ binding and disulfide bond formation.Phylogenetic analysis indicated that MnP1 and MnP2 of V.volvacea are close to the MnP1 ofLentinula edodes,but MnP3 and MnP4 are closely related to the peroxidase of Coprinopsis cinerea.The vv-mnpl,vv-mnp3 and vv-mnp4 genes were transcribed by the fungus during the mycelial growth by using RT-PCR.However,the V.volvacea MnP activity was not detected,indicating that V.volvacea MnPs lacked of the ability to oxidize Mn2+ to Mn3+.
NETL
NSTL
万方数据
维普
