Optimization of fermentation medium and intracellular polysaccharide extraction technique of Pleurotus pulmonarius
The culture medium for Pleurotus pulmonarius fermentation was optimized by the single factor experiment of carbon source and nitrogen source and the orthogonal experiment.P.pulmonarius intracellular polysaccharide extraction technique was optimized by using extraction rate as index in the orthogonal experiment with three factors,extraction duration,temperature and liquid-solid ratio.The results showed that the appropriate submerged fermentation culture medium of P.pulmonarius included saccharose 1.5％,bran 5％,peptone 0.6％,KH2PO4 0.15％,MgSO4 0.75％ and VB1 0.01％.The highest extraction rate was obtained on condition that liquid and solid ratio was at 50∶1 and extracting duration 2h at 90℃.Under such a condition,the polysaccharide extraction rate was 34.35％.
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万方数据
维普
