Comparison of the polysaccharides from fruiting bodies, mycelia and spore powder of Ganoderma lingzhi
The differences of polysaccharides extracted from fruiting bodies,mycelia and spore powder of Ganoderma lingzhi were compared.Polysaccharide concentration was determined by phonel-sulfate method,and monosaccharide compositions of the polysaccharides were analyzed by ion chromatography-integrated pulsed amperometric detector.HPLC spectrums of various polysaccharides and their changes after treated with a-amylase and endo-1,3-β-glucanase were also compared.The results indicated that polysaccharide content of mycelia was 3.81％,being the highest,and that of spore powder and fruiting body were 1.8％ and 0.59％,respectively.Monosaccharide composition and molar ratio of the three polysaccharides from mycelia,fruiting bodies and spore powder were different.In fruiting body,glucose and galactose were the main monosaccharide,but glucose was the main monosaccharide in mycelia and spore powder.HPLC spectrums revealed that the peaks and molecular weights of the three polysaccharides were also different.Changes between HPLC spectrums of polysaccharides treated by endo-1,3-β-glucanase and a-amylase indicated the three polysaccharides had different structure.NO release experiment using RAW 264.7 macrophages revealed that both polysaccharides from mycelia and fruiting bodies showed good activity of stimulating macrophages to release NO,however the polysaccharide from spore powder showed relatively lower activity.The study demonstrated that the polysaccharides of these three materials varied significantly,thus they should be differentially applied in pharmaceutical health care products.
polysaccharides of Ganoderma lingzhihigh performance liquid chromatography (HPLC)monosaccharide compositionimmune activity
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NSTL
万方数据
维普
