Isolation of secondary metabolites with acetylcholinesterase inhibitory activity of endophytic fungus Pezicula sp.JR14 from Huperzia serrata
An endophytic fungus strain JR14 with acetylcholinesterase (ACHE) inhibitory activity from Huperzia serrata was identified as Pezicula sp.by morphological characteristics and internal transcribed spacer (ITS) sequence analysis.Four kinds of secondary metabolites were separated and purified from the ethyl acetate extract of the endophytic Pezicula sp.JR14.According to mass spectrometry,1H-NMR and 13C-NMR analyses,they were identified as behenic acid (1),himeic acid B (2),secalonic acid A (3) and palmitic acid (4).Ellman colorimetric test proved that himeic acid B (2) showed better AChE inhibitory activity with IC50 value of 205μg/mL (0.64mmol/L).
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万方数据
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