摘要
基于高通量测序方法对贵州贵阳花溪刺槐内生真菌物种多样性进行分析,共获得有效序列43 942条,聚类后共获得81个可操作单元(OTUs),分属4门13纲27目45科58属.其中,优势属为一分类地位未定属,其相对丰度为83.23％,次优势属为镰刀菌属Fusafium (11.81％)、拟船壳属Gloniopsis (1.38％)和螺旋聚孢霉属Clonostachys (0.72％),相对丰度小于0.5％的属高达50个以上.Shannon-Wiener指数为0.6835,Simpson指数为0.7071.经FUNGuild软件平台解析显示,刺槐内生菌包含有如下生态功能群:植物病原菌、动物病原菌、内生真菌、真菌寄生菌、地衣真菌、粪生真菌、未定腐生菌和木腐菌等.
Abstract
Based on high-throughput sequencing technology,species diversity of the endophytic fungi from Robinia pseudoacacia in Huaxi District,Guiyang City,Guizhou Province were analyzed.The results showed that there were 43 942 valid sequences.Based on cluster similarity analysis 81 OTUs were obtained,belonging to 4 phyla,13 classes,27 orders,45 families,and 58 genera.Among these fungi,the dominant was the unclassified genus,and its relative abundance was 81.11％ of the total isolates.The next was Fusarium (11.81％),Gloniopsis (1.38％) and Clonostachys (0.72％),while the relative abundances of more than 50 genera were less than 0.5％.The Shannon-Wiener's diversity index and the Simpson's index of the endophytic fungi in the samples were 0.6835 and 0.7071 respectively.FUNGuild software platform analysis displayed that endophytic fungi of Robinia pseudoacacia contained the following functions' groups:plant pathogen,animal pathogen,endophyte,fungal parasite,lichen parasite,dung saprotroph,undefined saprotroph fungi and wood saprotroph.
国家科技期刊平台
NSTL
维普
万方数据