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西宁市萝卜黑心病的病原

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萝卜黑心病是近年来在青海省西宁地区发生的一种新病害,重病田发病率高达100%,严重影响了萝卜的产量和品质.罹病萝卜肉质根的根尖及毛根颜色变暗,纵向剖开病根,可见肉质根的中柱组织自根尖向上呈不同程度的黑色;横向剖开病根,可见肉质根的中柱组织呈现均匀或不均匀放射状分布的黑色变色点.经病原菌分离培养、科赫氏法则证病,及病原菌形态学和分子生物学鉴定,明确该病的致病菌包括长孢轮枝菌Verticillium Iongisporum、三体轮枝菌V.tricorpus 和瓜小织球壳菌Plectosphaerella cucumerina,其中长孢轮枝菌的组织侵染率达100%,为优势病菌.这3种病菌的适宜生长温度分别为20℃、20-25℃和25℃.本文是这3种病菌为害萝卜的首次报道,萝卜是这3种病菌的新寄主植物.
Pathogens of root internal discoloration (black heart disease) in radish in Xining City of Qinghai Province, China
A new disease of radish,black heart disease,has become increasingly severe in Xining City of Qinghai Province.The disease incidence could reach 100% in seriously infected fields.Through pathogen isolation and culture,and Koch's postulate test,the pathogens were clarified.Based on morphological and molecular biological characters,the pathogens were identified as Verticillium Iongisporum,V.tricorpus and Plectosphaerella cucumerina.The optimum growing temperatures for these three species were 20℃,20-25℃ and 25℃,respectively.Infection ratio of V.Iongisporum reached 100% in tested samples.This is the first report of the three pathogens causing radish black heart disease,and Raphanus sativus is a new natural host for these three fungal pathogens.

Raphanus sativusblack heartVerticillium longisporumVerticillium tricorpusPlectosphaerella cucumerina

苗增建、何苏琴、张晓梅、文朝慧、白滨、孔小平

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青海省西宁市蔬菜研究所 青海西宁810016

甘肃省农业科学院植物保护研究所 甘肃兰州730070

甘肃出入境检验检疫局 甘肃兰州730020

甘肃省农业科学院农业质量标准与检测技术研究所 甘肃兰州730070

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萝卜 黑心病 长孢轮枝菌 三体轮枝菌 瓜小织球壳菌

甘肃省农业科技创新项目2016年青海省高端人才千人计划项目青海省基础研究计划项目

GNCX-2014-342017-ZJ-745

2018

菌物学报
中科院微生物研究所 中国菌物学会

菌物学报

CSTPCDCSCD北大核心
影响因子:1.187
ISSN:1672-6472
年,卷(期):2018.37(4)
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