Pathogens of root internal discoloration (black heart disease) in radish in Xining City of Qinghai Province, China
A new disease of radish,black heart disease,has become increasingly severe in Xining City of Qinghai Province.The disease incidence could reach 100% in seriously infected fields.Through pathogen isolation and culture,and Koch's postulate test,the pathogens were clarified.Based on morphological and molecular biological characters,the pathogens were identified as Verticillium Iongisporum,V.tricorpus and Plectosphaerella cucumerina.The optimum growing temperatures for these three species were 20℃,20-25℃ and 25℃,respectively.Infection ratio of V.Iongisporum reached 100% in tested samples.This is the first report of the three pathogens causing radish black heart disease,and Raphanus sativus is a new natural host for these three fungal pathogens.