Composition and diversity of culturable endophytic fungal community in roots of two larch species in Inner Mongolia
Three forest regions of Larix gmelinii and four forest regions of L.gmelinii var.principis-rupprechtii in Inner Mongolia were selected as sampling points.Endophytic fungi in root segments of the two larch species were isolated and cultured by tissue isolation methods,and identification of the fungi was based on morphological characteristics and rDNA ITS region sequence analysis.The community composition and diversity of root endophytic fungi in different tree species and different plots of the same tree species were analyzed.The results were that 225 isolates of culturable endophytic fungi were obtained,belonging to 31 species of 17 genera in 9 families of 4 orders and 3 classes.Among them,dark septate endophytes(DSE)accounted for the largest proportion and were the main endophytic fungal group.Cadophora echinata,Phialocephala fortinii,Phi.helvetica and Pleotrichocladium opacum were in common in the roots of the two larch species.Among them,Phi.fortinii and Phi.helvetica were dominant in the roots of L.gmelinii and L.gmelinii var.principis-rupprechtii respectively.The root-specific endophytic fungal species in the two larch species were different,and the similarity of community structure was low.The diversity index and evenness index of endophytic fungi in L.gmelinii var.principis-rupprechtii were higher than those in L.gmelinii.The evenness of fungal community in the three sample plots of L.gmelinii forest region was low.The similarity coefficient between different sites of the same tree species indicated that the community composition was comparatively similar when the geographical location and stand conditions were similar.In conclusion,there were some differences in the composition of endophytic fungi in the roots of the two larch species,and the fungal community structure of the same tree species in different plots were obviously different,but overall,endophytic fungal resources in roots of larches in Inner Mongolia were very rich.
万方数据
维普
