Diversity and community structure of epiphytic fungi in the phyllosphere of Rosa chinensis in Beijing,northern China
Phyllosphere microorganisms play important roles in ecological functions,such as maintaining plant health,promoting plant growth and biogeochemical cycling.In recent years,the role and importance of phyllosphere microbes have received increasing attention.The diversity and community structure of epiphytic fungi in the phyllosphere of Rosa chinensis,a common greening plant in Beijing,were investigated.Four sites from the center to north of the city and near to far distance,Andingmen area(ADM),Hepingjie area(HPJ),Yayuncun area(YYC),and Beiqijia area(BQJ),were selected.The samples were taken in three seasons(April,July,and November)at different locations(the middle and sides of the road of the selected areas).A total of 3 425 amplicon sequence variants(ASVs)was obtained,and they were dominated by Dothideomycetes.The variance of Shannon index between sampling sites were ADM>BQJ>HPJ>YYC,indicating ADM is significantly higher than YYC.The diversity shows a decreasing and then increasing trend from the center to north of the city.The variance of Shannon index between seasons showed April>July>November,and April was significantly higher than July and November.There is no significant difference between the samples from the center and the sides of road.LEfSe analysis suggested the significant biomarkers of different regions and seasons.Significant biomarkers of Andingmen area were Neosetophoma,Neophaeococcomyces and Pseudocosmopora,while the significant biomarkers of Hepingjie area,Yayuncun area and Beiqijia area were Preussia,Orbilia and Pseudotomentella,respectively.Network analysis showed the dominant genera were Vacuiphoma,Erythrobasidium,Cladosporium,Mrakia,and Sphaerulina.The main functional groups of epiphytic fungi in the phyllosphere of Rosa chinensis were pathotroph and saprotroph.The prediction of fungal community function demonstrates that saprophytic functional groups were different in different seasons and sampling sites.The study revealed structural changes of phyllosphere fungi in urban plants and might be valuable to scientific management of urban greening.
万方数据
维普
