Function analysis of Ganoderma applanatum FIP-gap1 under temperature stress
FIP-gap1 is a fungal immunomodulatory protein(FIP)identified from Ganoderma applanatum,which is a typical member of Ganoderma FIPs.However,its native biological function towards G.applanatum and its resistance under temperature stresses remain unclear.In this study,Pichia transformation,qRT-PCR test,mycelial culture and physiological and biochemical indexes examination of FIP-gap1 were carried out.The qRT-PCR test showed that FIP-gap1 expression significantly increased under temperature stress,and its relative expression level at 36℃was 8.9 fold increase over that under normal conditions;yeast cell survival and spot test showed that FIP-gap1 gene-transformed Pichia pastoris grew significantly better than the wild-type strains at 36℃.The growth rate of FIP-gap1-overexpressed G.applanatum(pGPD:FIP-gap1)was significantly higher than that of wild-type(CK).Besides,soluble saccharides and activities of SOD,CAT and APX in pGPD:FIP-gap1 were higher than those in wild-type,whereas content of MDA in pGPD:FIP-gap1 was lower than that of wild-type.In conclusion,the FIP-gap1 gene can affect the mycelial growth and respond to high temperature stress,and thereby improve the stress tolerence of yeast and G.applanatum under temperature stresses.For the first time,the native biological function of FIP-gap1 towards G.applanatum and its response to temperature stress were clarified,and this study provides theoretical basis for further studying fungal FIPs,as well as the application and development of FIP-gap1.
万方数据
维普
