Function of the key transcription factor CfCrzA in the pathogenesis of Colletotrichum fructicola
Glomerella leaf spot caused by Colletotrichum fructicola is a new disease of apple in China.CfCrzA,a transcription factor expressed in the early stage of infection,has been previously found through omics analysis.The present study intends to clarify its role in the growth,development and pathogenicity of C.fructicola.Bioinformatics analysis showed that CfCrzA was a conserved gene in the Colletotrichum genus.Two deletion mutants of CfCrzA were obtained using homologous recombination.According to phenotypic analysis,Δ CfCrzA mutants had no significant difference in growth rate from wild type WT.The pathogenicity of Δ CfCrzA on apple leaves and fruits decreased significantly.Inoculation test on cellophane paper showed that the spore germination rate of Δ CfCrzA decreased by 26%±3%,and the appressorium formation rate reduced by 43%±1%.Leaf histological observation indicated that the appressorium formation rate of Δ CfCrzA decreased by 60%±1%,but the formation of infection vesicles and primary hyphae was not affected.Culture observation revealed that the sensitivity of Δ CfCrzA to cell wall stress-related SDS increased significantly.These results demonstrate that the transcription factor CfCrzA is a key pathogenic factor for C.fructicola,mainly influencing its pathogenicity through affecting conidial germination and appressorium formation.
万方数据
维普
