Bioinformatics analysis and expression changes during the subculture process of the sod gene of Volvariella volvacea
Superoxide dismutase is the core enzyme in the antioxidant system.In this study,the physicochemical properties,conserved structural domains and evolutionary relationships of the protein encoded by the sod gene of Volvariella volvacea were predicted by bioinformatics analysis,and the physiological characters of mycelium and the relative expression of sod genes during the subculture of Volvariella volvacea were determined.The results showed that the genome of Volvariella volvacea contained one Cu/Zn-sod gene and three Mn-sod genes,and all of them encoded proteins with typical characteristics of SOD,belonging to the SOD family members.The four SODs of Volvariella volvacea had high homology with SODs of different fungi.The relative expressions of Cu/Zn-sod and Mn-sod3 genes increased first and then decreased during subculture.The relative expressions of Mn-sod1 gene were higher than T0,while those of Mn-sod2 were lower than T0.This study lays the foundation for further revealing the biological function of sod gene in Volvariella volvacea and the mechanism of subculture degeneration of the strain.
万方数据
维普
