Function and interacting proteins of effector Ch4839 in Colletotrichum horii
Persimmon anthracnose is one of the main diseases affecting cultivation and industrial development of persimmon.The main fungal pathogen of persimmon anthracnose in China is Colletotrichum horii.The effector is a type of secreted protein by pathogenic microbes to induce or inhibit host defense response.At present,there are few studies on the effector of C.horii.In this study,the effector Ch4839 was screened from the genome of C.horii,and its function and target protein(s)screened were analyzed.The results showed that ΔCh4839-4-1,the knockout mutant of Ch4839,did not affect the colony growth of C.horii but the sporulation rate was reduced.The pathogenicity of the knockout mutant decreased significantly.Subcellular localization showed that the effector was localized in the nucleus,cell membrane and some cytoplasm of tobacco.In addition,Ch4839 exhibited the inhibition of the programmed cell death of tobacco-induced by INF1,indicating that the effector possessed a role in inhibiting host defense.There were 5 candidate target proteins obtained by yeast library screening.The results preliminarily reveal the function of the effector Ch4839,and further exploration of the interaction mechanism between the effector and the host target protein is needful.
Colletotrichum horiianthracnose of persimmoneffector knockoutyeast two-hybird
万方数据
维普
