Extracts of Sanghuangporus vaninii inhibit SARS-CoV-2 and inflammation
The inhibitory effects of different polar extracts of Sanghuangporus vaninii(water,ethanol,petroleum ether,ethyl acetate,n-butanol,and extraction residual water extracts)on the production of inflammatory cytokines IL-6 and TNF-α in RAW264.7 cells stimulated by LPS were evaluated.The inhibition of SARS-CoV-2 replication and entry by ethanol and water extract of S.vaninii was appraised.The expression level of nucleocapsid protein was detected by real-time fluorescent quantitative PCR and Western Blot using Vero as host cells.The results indicated that the ethanol,petroleum ether,ethyl acetate,n-butanol,and water extracts of S.vaninii showed good anti-inflammatory effects,and the inhibitory effect was significantly enhanced with the increase of concentration,and the levels of IL-6 and TNF-α produced by RAW264.7 cells stimulated by LPS were significantly reduced.The ethanol extract and water extract of S.vaninii significantly inhibited viral replication by inhibiting the expression level of nucleocapsid protein in SARS-CoV-2.
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