Effects of single and mixed metal ions on laccase activities of Pleurotus ostreatus CY 568 in submerged fermentation
The effects of single and mixed Cu2+,Fe2+ and Mn2+ on the activities of laccase secreted by Pleurotus ostreatus CY 568 were investigated.The results showed that single and mixed metal ions had significant effects on laccase activity of P.ostreatus CY 568(P<0.001).The presence of Cu2+ or Mn2+,especially Cu2+,was helpful for improving laccase activity of P.ostreatus CY 568.Stable high laccase activity was maintained in medium with Cu2+.Compared with the basal medium,the medium with the mixture of metal ions could enhance the laccase activity of P.ostreatus CY 568,and the maximum laccase activity reached(22 622.22±142.97)U/L after fermentation on a medium mixed with Cu2+ and Mn2+(MMCM)for 18 days.The maximum laccase activity of P.ostreatus CY 568 in the medium containing Cu2+ was higher than that in the medium without Cu2+,indicating that Cu2+ contributed to the improvement of the laccase activity of P.ostreatus CY 568.Laccase activity of P.ostreatus CY 568 fermented in medium with single Fe2+ was lower than that in basal medium,indicating that Fe2+ could inhibit the laccase activity of P.ostreatus CY 568.
Pleurotus ostreatussingle metal ionmixed metal ionslaccase activity
万方数据
维普
