Analyses of components and main pathways involved in fruiting body development of Pleurotus ostreatus based on untargeted metabolomics
In order to explore the underlying mechanism of fruiting body formation and development of Pleurotus ostreatus,the small molecular metabolites in primordia and newly differentiated fruiting bodies were investigated through metabolomics approaches.All the 545 metabolites including phenolic acids,lipids,amino acids and their derivatives,nucleotides and their derivatives,organic acids,alkaloids,tannins,lignans and coumarins,and others were identified.Principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)revealed a significant difference between metabolites of primordia and newly differentiated fruiting bodies.A total of 253 significantly differential metabolites was screened and identified under the conditions of VIP(varible importance in the projection)≥1 and fold change≥2 or≤0.5,involving in 76 pathways.It was inferred that the deep transformation and regulation of components were accomplished via tryptophan metabolism,phenylalanine,tyrosine and tryptophan biosynthesis,lysine degradation,D-arginine and D-ornithine metabolism,biotin metabolism,tropane,piperidine and pyridine alkaloid biosynthesis,tyrosine metabolism,purine metabolism,propanoate metabolism and isoquinoline alkaloid biosynthesis pathways.This study will provide a theoretical basis for exploring the developmental mechanism of Pleurotus ostreatus fruiting body.
Pleurotus ostreatusfruiting body developmentmetabolomicsdifferential metabolitesmetabolic pathway
万方数据
维普
