Effects of lotus-seed-husk substrate on Ganoderma lingzhi growth and production benefits
As an agricultural byproduct,the husks of lotus seeds can be used to cultivate edible fungi.This study aims at utilizing lotus seed husks to cultivate Ganoderma lingzhi in order to reduce the costs and increase the efficiency of cultivation.The 10%,20%,40%,60%and 80%lotus seed husks were used to replace sawdust and corncob in the basic formula(45%sawdust,45%corncob,5%wheat bran,4%corn flour,and 1%gypsum)of conventional cultivating substrate.The agronomic traits,nutrient content and production benefits were investigated to select the high-efficient formula.Results showed that certain proportion of lotus seed husks in the substrate could promote the mycelium growth rate of G.lingzhi.The growth rate reached 4.44 mm/d under the replacement of 60%lotus seed husks.Ganoderma lingzhi cultivated in the lotus-seed-husk substrate displayed shorter stipe,thinner cap and lower moisture content of basidiomata,while the cap size,stipe diameter and fresh weight of basidiomata showed no significant difference.The determination of nutrients revealed that the polysaccharide and protein content in basidiomata significantly increased with the increase of lotus seed husk proportion in the substrate.The polysaccharide content in basidiomata cultivated in the substrate with 80%lotus seed husks(10.57 mg/g)was 1.66 times higher than that in the conventional substrate,and the protein content(15.14%)was also significantly higher than that in basidiomata cultivated in the conventional substrate(14.12%).However,the triterpenoid acids displayed a trend of"low proportion promotion,high proportion inhibition"in the substrate with lotus seed husk replacement.Besides,lotus-seed-husk substrate significantly improved total amino acid content in G.lingzhi basidiomata,particularly in the substrate with 10%lotus seed husks(32.44%).In total,13 amino acids were detected.Production benefit analysis showed that G.lingzhi cultivated in lotus seed husk substrate significantly improve the cultivation profit,and the profit was the highest(1.22 yuan per bag)when cultivation was carried out in the substrate with 80%lotus seed husks.