Extraction and antioxidant activities of selenium polysaccharide from selenium-enriched Auricularia heimuer mycelia
In order to obtain the best preparation process of selenium polysaccharide from selenium-enriched Auricularia heimuer mycelium,A.heimuer strains Jiufeng,Heifeng and Xizang 6 were used as materials,and selenium tolerance and gradient concentration selenium enrichment screening,single factor test,response interview design analysis,and antioxidant determination were carried out for extraction and process-optimization of selenium polysaccharide from the mycelia.Antioxidant determination method was used to examine antioxidant activity of selenium polysaccharide.The results showed that'Jiufeng'had the strongest selenium tolerance among the three A.heimuer strains.Selenium enriched fermentation experiment indicated that the total selenium content of A.heimuer mycelia was the highest when the selenium concentration was 60 μg/mL.The optimal conditions for selenium polysaccharide extraction were:ultrasonic time of 25.4 min,water bath time of 56 min,and material to liquid ratio of 1:49(g:mL).Under this condition,the extraction rate of selenium polysaccharide was(17.49±0.10)%.Selenium-enriched A.heimuer mycelial polysaccharide has certain scavenging ability to DPPH and hydroxyl radicals,and shows strong reducing ability in the total reducing power test.The research results provide a preliminary theoretical basis for the selenium enriched culture of A.heimuer strains,and extraction of selenium polysaccharides from A.heimuer mycelia.
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