Effects of different phosphorus fertilizer levels and intercropping on soil fungal community in wheat field
The response to phosphorus fertilizer level and planting pattern of soil fungal community in wheat field was analyzed.Three phosphorus(P)application rations,90 kg/hm2(P1),135 kg/hm2(P2)and 180 kg/hm2(P3),and two planting modes mono-cropping(WS)and intercropping(WI),were set.Illumina high-throughput sequencing was used to investigate the effects of different phosphorus fertilization levels and intercropping on soil nitrogen and phosphorus content and fungal community composition and diversity in the field of winter wheat.The results showed that different phosphorus application levels had significant effects on soil phosphorus content in intercropping and mono-cropping wheat fields.Intercropping was beneficial to increase available phosphorus content of the soil with low phosphorus level.In total,1 157 759 effective tags and 847 OTUs were obtained by high-throughput sequencing.All the treated OTUs were classified into 8 phyla,26 classes,54 orders,82 families and 108 genera.There were 196 OTUs in 6 treatments.There were 147 and 102 unique OTUs in intercropping and monoculture modes,respectively.There were 113,70 and 66 OTUs unique to P1,P2 and P3 treatments,respectively.Phosphorus application and intercropping had little effect on fungal alpha diversity in wheat rhizosphere soil.The results of PCoA analysis showed that the level of phosphorus application and intercropping and their interaction had certain effects on fungal community structure.The results of redundancy analysis showed that soil available phosphorus had a greater impact on the fungal community structure,and Ascomycota,Basidiomycota,Mortierellomycota and Glomeromycota were positively correlated with available phosphorus,and Chytridiomycota was negatively correlative.
winter wheatfungal community structureIllumina high-throughput sequencingphosphate fertilizer levelintercropping
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万方数据
