In vitro digestion and fermentation characteristics of Clitocybe squamulosa polysaccharides extracted by ultrasound-assisted deep eutectic solvents
This study aims at investigating the effects of in vitro digestion and fecal fermentation on the chemical composition,structural characteristics and biological activities of Clitocybe squamulosa polysaccharides(D-CSFP)extracted by ultrasound-assisted deep eutectic solvents.The results showed that after saliva,stomach and intestine digestion,the content of D-CSFP increased from 0.44 mg/mL to 0.45,0.55 and 0.57 mg/mL respectively and the molecular weight of D-CSFP decreased from 35 312 Da to 32 090,25 519 and 25 473 Da respectively,but the structure of D-CSFP had no obvious change.After fecal fermentation in vitro,the OD600 of D-CSFP fermentation broth increased first and then decreased(0.63→ 1.24→1.19);pH decreased from 6.75 to 6.13(P＜0.05);the total carbohydrate content in D-CSFP fermentation broth decreased from 8.59 mg/mL to 5.05 mg/mL(P＜0.05);the total short chain fatty acids(SCFA)content was significantly increased from 1.83 mmol/L to 11.15 mmol/L(P＜0.05).16S rRNA sequencing results showed that D-CSFP could effectively promote the relative abundance of beneficial bacteria(such as Bacteroides,Prevotella and Dialister),and inhibit the propagation of harmful bacteria(such as Klebsiella).In conclusion,D-CSFP can be used as a potential functional food to promote intestinal health.
Clitocybe squamulosa polysaccharidesdigestion and fermentationshort chain fatty acidsintestinal flora
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